Part:BBa_K2813002:Design

SaCas9 without gRNA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1975
  Illegal BglII site found at 2128
  Illegal BglII site found at 2274
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1548
  Illegal AgeI site found at 4104
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

One pot mutagenesis has to be designed for certain sites to elicit protein inactivation.

Source

This part is a nuclease enzyme that is derived from Streptococcus aureus.